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Detection of Chlamydia trachomatis by plasmid Gap-LCR-ELISA
Author(s): 
Pages: 148-150
Year: Issue:  3
Journal: JOURNAL OF CLINICAL DERMATOLOGY

Keyword:  衣原体沙眼质粒缺口-连接酶链反应-酶联免疫吸附法;
Abstract: 目的:建立高度灵敏特异的质粒缺口-连接酶链反应-酶联免疫吸附法(Gap-LCR-ELISA)检测沙眼衣原体(Chlamy-dia trachomatis,CT).方法:根据CT共有隐蔽性质粒设计并标记4条寡核苷酸探针,对6型CT标准株和鹦鹉热衣原体及其他常见泌尿生殖道病原菌行Gap-LCR-ELISA.结果:质粒Gap-LCR-ELISA可检测出6型CT,并与其他病原体无交叉反应,其最低检测限为2.5个原体(elementary bodies,EB),比PCR敏感10倍.结论:质粒Gap-LCR-ELISA对检测CT感染具有极高的灵敏度和特异度,适宜于我国进行大规模CT流行病学调查.
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