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Studies on cisplatin induced apoptosis in mice melanoma Cloundman S91 cells
Author(s): 
Pages: 6-8
Year: Issue:  1
Journal: JOURNAL OF CLINICAL DERMATOLOGY

Keyword:  黑素瘤Cloundman S91细胞株细胞凋亡顺铂小鼠;
Abstract: 目的:探讨顺铂(CDDP)对小鼠黑素瘤Cloundman S91细胞株的作用.方法:采用四甲基偶氮唑蓝(MTT)还原法检测CDDP对S91细胞株存活率的影响,吖啶橙染色观察凋亡细胞形态,流式细胞仪分析凋亡细胞周期,末端脱氧核苷转移酶(TdT)介导的脱氧尿苷三磷酸(dUTP)末端标记法(TUNEL)原位观察,计算细胞平均凋亡指数.结果:S91细胞经CDDP处理后,存活率降低,并与药物浓度呈正相关;荧光显微镜下可见明显的凋亡细胞;细胞周期G1期前出现凋亡峰.CDDP浓度在10、20、30μmol/L时,细胞平均凋亡指数分别为11.5%、19.8%、33.7%.结论:10~30μmol/L的CDDP诱导S91黑素瘤细胞凋亡.
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