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Effect of 80MeV/u 20Ne10+ Ions on DNA Damage and Cell Cycle of SMMC-7721 Cells
Author(s): 
Pages: 341-345
Year: Issue:  5
Journal: ACTA LASER BIOLOGY SINICA

Keyword:  重离子SMMC-7721细胞DNA损伤细胞周期流式细胞术;
Abstract: 从辐照剂量和修复时间两个角度研究了重离子辐照对肿瘤细胞DNA损伤及细胞周期的影响,为重离子治癌的临床应用积累基础数据.不同剂量的80MeV/u20Ne10+辐照SMMC-7721细胞样品,利用单细胞凝胶电泳技术(Single Cell Gel Electrophoresis,SCGE)对细胞DNA损伤进行了检测,利用流式细胞技术(Flow Cytometry Methods,FCM)对细胞周期变化进行了分析.80MeV/u20Ne10+辐照后4小时内,SMMC-7721细胞的DNA损伤与辐照剂量呈线性关系,在0小时组其线性相关因子r为0.9621,4小时组为0.914;随着修复时间的增加,DNA损伤与辐照剂量不再线性相关,但0.5Gy, 1Gy和2Gy三个剂量点的DNA损伤程度极为相近.另外,重离子辐照后SMMC-7721细胞发生S期和G2/M期阻滞现象,其随剂量变化及时间变化的规律不同于X、γ等低LET(Linear Energy Transfer)射线辐照.
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