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Noise exposing induced mitochondrial DNA deletions in rats
Author(s): 
Pages: 324-328
Year: Issue:  5
Journal: CHINESE JOURNAL OF OTORHINOLARYNGOLOGY

Keyword:  DNA线粒体基因缺失听觉丧失噪声性聚合酶链反应;
Abstract: 目的探讨线粒体DNA(mitochondrial DNA,mtDNA)缺失与噪声性聋的关系.方法 3月龄大鼠分噪声暴露组和无暴露对照组,各20只.噪声暴露组暴露于108~110 dB SPL的白噪声 4 h/d,共40 d;对照组不接触噪声.噪声暴露结束2周后测试两组大鼠的听性脑干反应(auditory brainstem response,ABR)阈值,聚合酶链反应(polymerase chain reaction,PCR)检测其耳蜗、蜗核、颞叶脑组织和颞肌组织中是否存在mtDNA4834缺失,对存在的缺失进行定量分析;PCR产物进行克隆、测序.结果噪声暴露组大鼠出现永久性阈移,其ABR平均阈值(±s,下同)为(75.25±6.17)dB SPL (40耳),对照组为(34.75±3.80)dB SPL(40耳),两组ABR阈值的差异具有非常显著性意义(P<0.01).噪声暴露组大鼠的耳蜗、蜗核、颞叶脑组织中mtDNA4834缺失发生率(24/40、16/20、18/20)明显高于对照组,两组之间的差异具有非常显著性意义(P<0.01);而与听觉无关的颞肌组织中mtDNA4834缺失发生率(2/20)在两组中均很低,两组之间的差异无显著性(P>0.05);噪声暴露组大鼠的耳蜗、蜗核、颞叶脑组织和颞肌中mtDNA4834缺失占总mtDNA的平均百分率(±s)分别为(0.998 8±0.551 6)×10-2%、(1.060 9±0.672 0)×10-2%、(1.206 0±0.687 5)×10-2%和(0.206 4±0.142 7)×10-2%,其中颞肌组织最低.结论长期噪声暴露可引起耳蜗、蜗核及颞叶脑组织mtDNA4834缺失;mtDNA4834缺失与噪声性聋的发病有关.
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