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The properties of potassium currents in isolated Hensen cells and effects of ATP on ion currents
Author(s): 
Pages: 343-346
Year: Issue:  5
Journal: CHINESE JOURNAL OF OTORHINOLARYNGOLOGY

Keyword:  迷路支持细胞腺苷三磷酸舒拉明四乙基铵膜片钳术;
Abstract: 目的研究豚鼠耳蜗单离Hensen细胞的钾离子电流及其特性以及三磷酸腺苷(adenosine triphosphate,ATP)对Hensen细胞电生理特性的影响.方法采用传统全细胞膜片钳技术,对单离Hensen细胞进行记录.ATP通过压力注射仪对单离Hensen细胞给药.结果 Hensen细胞的钾电流呈明显的外向整流性,只有延迟整流性钾电流(delayed rectification potassium current,IK),没有瞬间外向性钾电流(transient outward potassium current,IA).低浓度(0.1 μmol/L,1 μmol/L,10 μmol/L)ATP可以使Hensen细胞的钾电流的幅度明显降低,且呈浓度依赖性,浓度越高,降幅越大.高浓度ATP(100 μmol/L,1 mmol/L,10 mmol/L)可以引起Hensen细胞的内向离子流,呈浓度依赖性,浓度越高,升幅越大.ATP的这两种作用均可被ATP受体拮抗剂(100 μmol/L舒拉明)所逆转.结论对Hensen细胞不同电压的刺激可以诱发出延迟整流性钾电流,低浓度ATP对Hensen细胞的钾电流有明显抑制作用,且呈浓度依赖性.高浓度ATP可以引起Hensen细胞的非选择性内向离子流,为钾离子依赖性,而且是通过ATP受体起作用.
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