The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Bookmark and Share
Studies on Fermentation Conditions for Penicillin G Acylase Production with a Recombinant Bacillus subtilis System
Author(s): 
Pages: 266-271
Year: Issue:  3
Journal: JOURNAL OF CHEMICAL ENGINEERING OF CHINESE UNIVERSITIES

Keyword:  青霉素G酰化酶温度诱导重组枯草杆菌发酵条件;
Abstract: 对产青霉素G酰化酶的温度诱导型重组枯草芽孢杆菌发酵条件进行了研究.结果表明,培养基的最佳氮源和碳源组成为:35g·L-1牛肉膏、3g·L-1葡萄糖和6g·L-1淀粉;最佳诱导时机是细胞对数生长的中后期,诱导前应将pH调节至中性;最佳诱导条件为升高温度至50℃并维持4min;随后将温度降低到34℃进行重组蛋白的表达.在上述条件下,PGA的表达水平可以达到5.8U·mL-1.SDS-PAGE电泳分析表明该重组菌能够将绝大部分表达的PGA分泌到细胞外,而且表达的PGA蛋白占有高比例(≥90%).
Related Articles
No related articles found