Author(s): WANG Li, SUN Si-tong, ZHANG Wen-jun, LIANG Shuang, REN Jun-gang, School of Pharmacy, Harbin University of Commerce
Pages: 15-20
Year: 2020 Issue:  1
Journal: Central South Pharmacy
Keyword:  puerarin; phospholipid complex; nanoparticle; PBCA; in vitro release;
Abstract: Objective To prepare puerarin phospholipid complex nanoparticles with polycyanoacrylate（PBCA） as the carrier material and evaluated in vitro.Methods Nanoparticles were prepared by interfacial condensation polymerization.The recombination rate,encapsulation efficiency and drug loading were used as evaluation indexes,the formulation and process were optimized by single factor and orthogonal test.Results The best prescription and process of puerarin phospholipid complex:included anhydrous ethanol as the reaction solvent,the ratio of puerarin to phospholipid at 1∶2.The best prescription and process of puerarin phospholipid complex nanoparticles were:pH= 3.0,the concentration of alpha-cyanoacrylate（α-BCA） at 0.8%,Voil phase∶ Vwater phase= 1∶60,and the dosage of puerarin phospholipid complex was 1.0 mg.The average particle size of the prepared nanoparticles was（115.1±3.45） nm,the encapsulation rate was（90.03±1.80）%,and the drug loading was（11.80±0.12）%.The cumulative release of in vitro release at 24 h was approximately 80%.Conclusion The prepared puerarin phospholipid composite nanoparticles show sustained-release feature in vitro with high encapsulation and drug loading.