Bioinformatics Analysis of PuerarinTreating Osteoporosis
Author(s): LIANG Xuezhen, LUO Di, XU Bo, LIU Jinbao, LI Gang, Shandong University of Traditional Chinese Medicine, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine
Pages: 2628-2631+2824
Year: 2019 Issue: 11
Journal: Chinese Archives of Traditional Chinese Medicine
Keyword: osteoporosis; puerarin; network pharmacology; bioinformatics; GEO database;
Abstract: Objective: The key molecular mechanism of puerarin in the treatment of osteoporosis was preliminarily analyzed to provide new potential targets in treating osteoporosis based on bioinformatics and network pharmacology. Method: We searched the related microarray of puerarin and osteoporosis in GEO database, and analyzed the differentially expressed genes by R language, and constructed the protein-protein interaction(PPI) of differentially expressed genes, and screened the specific targets of puerarin in treatment of osteoporosis. Go enrichment analysis and KEGG pathway analysis of key differentially expressed genes were performed using DAVID database. Result:①The puerarin has good drugability and the value is 0.69.②We retrieved the GEO database to determine the chip with puerarin correlation as GSE85871 and with osteoporosis correlation as GSE56116 and 667 differential genes and 1228 differential genes were screened by R language analysis respectively.③The GeneMANIA database was used to construct PPI, and 80 key genes were merged by Cytoscape. ④The key differentially expressed genes were analyzed by functional annotation of DAVID database,which included apoptotic process, androgen receptor signaling pathway, cell division, response to calcium ion and positive regulation of transcription, DNA-templated and so on, and covered Protein processing in endoplasmic reticulum, insulin signaling pathway, apoptosisand GnRH signaling pathway and so on. Conclusion: Bioinformatics and network pharmacology could be used to effectively analyze the original gene chip data of GEO database, which could not only identify the known signal pathways related to the treatment of osteoporosis with puerarin, but also find out some new pathways or biological processes.Puerarin is expected to be a new molecule in the treatment of osteoporosis.
Pages: 2628-2631+2824
Year: 2019 Issue: 11
Journal: Chinese Archives of Traditional Chinese Medicine
Keyword: osteoporosis; puerarin; network pharmacology; bioinformatics; GEO database;
Abstract: Objective: The key molecular mechanism of puerarin in the treatment of osteoporosis was preliminarily analyzed to provide new potential targets in treating osteoporosis based on bioinformatics and network pharmacology. Method: We searched the related microarray of puerarin and osteoporosis in GEO database, and analyzed the differentially expressed genes by R language, and constructed the protein-protein interaction(PPI) of differentially expressed genes, and screened the specific targets of puerarin in treatment of osteoporosis. Go enrichment analysis and KEGG pathway analysis of key differentially expressed genes were performed using DAVID database. Result:①The puerarin has good drugability and the value is 0.69.②We retrieved the GEO database to determine the chip with puerarin correlation as GSE85871 and with osteoporosis correlation as GSE56116 and 667 differential genes and 1228 differential genes were screened by R language analysis respectively.③The GeneMANIA database was used to construct PPI, and 80 key genes were merged by Cytoscape. ④The key differentially expressed genes were analyzed by functional annotation of DAVID database,which included apoptotic process, androgen receptor signaling pathway, cell division, response to calcium ion and positive regulation of transcription, DNA-templated and so on, and covered Protein processing in endoplasmic reticulum, insulin signaling pathway, apoptosisand GnRH signaling pathway and so on. Conclusion: Bioinformatics and network pharmacology could be used to effectively analyze the original gene chip data of GEO database, which could not only identify the known signal pathways related to the treatment of osteoporosis with puerarin, but also find out some new pathways or biological processes.Puerarin is expected to be a new molecule in the treatment of osteoporosis.
Related Articles