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Analysis of total DNA per cell on organism of chromomycosis by Flow Cytometry
Author(s): 
Pages: 494-495
Year: Issue:  8
Journal: JOURNAL OF CLINICAL DERMATOLOGY

Keyword:  着色芽生菌病致病菌流式细胞术DNA含量;
Abstract: 应用流式细胞术(FCM)比较4种7株着色芽生菌病致病菌间DNA含量的差异.研究用卡氏枝孢霉4株,疣状瓶霉、裴氏着色霉、紧密着色霉1株,制备稳定生长阶段各菌株单孢子悬液,碘化丙啶(PI)一步插入法荧光染色,用人淋巴细胞作为内参标准进行DNA含量测定,80%以上的细胞处于DNA合成周期的G0/G1期,统计学处理显示4种致病菌DNA含量两两比较存在显著性差异,而卡氏枝孢霉种内两两比较DNA含量无显著性差异.FCM有望为医学真菌学提供一种先进、有效的研究方法和途径.
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