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Expression of H19 in glioblastoma multiforme and its effect on cell proliferation and invasion
Pages: 521-525
Year: Issue:  6
Journal: Journal of International Neurology and Neurosurgery

Keyword:  Long noncoding RNAGlioblastoma multiformeInvasionProliferationApoptosis;
Abstract: 目的 探讨长链非编码RNA(Long noncoding RNAs,LncRNAs) H19在人多形性胶质母细胞瘤((glioblastoma mutliforme,GBM)中的表达及其对U87-GBM生物学功能的影响及作用机理.方法 实时荧光定量核酸扩增检测系统(Real-time Quantitative PCR Detecting System,qPCR)检测H19在GBM表达;CCK-8试剂盒(Cell Counting Kit-8,CCK-8)检测U87细胞增殖;流式细胞技术(Flow Cytometry,FCM)检测U87细胞凋亡及细胞周期;侵袭小室(transwell)检测U87细胞迁移和侵袭.蛋白质印迹法(Western blot)法检测P53表达.结果 H19在GBM中高表达,上调H19促进GBM的增殖、侵袭、迁移;抑制凋亡;增加了细胞周期S期比例;抑制了P53的表达.结论 H19促进GBM的致癌性.
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