Effects of Parthenolide and Decending Speed of Temperature on Chilling Resistance of Imbibed Seeds of Lactuca sativa
Author(s): SHEN Meng-qi, HAN Ying-ying, ZHOU Qiang, LI Wei-jie, LIU Bao-lin
Pages: 308-314
Year: 2016 Issue: 4
Journal: Subtropical Plant Science
Keyword: Lactuca sativa; seed; programmed cooling; cooling rate; chilling stress; Parthenolide;
Abstract: In order to study the effects of Parthenolide on chilling resistance of Lactuca sativa seeds during programmed cooling, the seeds were imbibed by exogenous animal ubiquitin E3 ligase activator Parthenolide before treated with programmed cooling with different cooling rates. The results showed that the addition of Parthenolide reduced the freezing tolerance of lettuce seeds in both fast and slow cooling treatments, and the germination rates after both cooling treatments were significantly lower than control, the effects of Parthenolide were the most obvious when the temperature was reduced to -20 ℃, which indicating that during the programmed cooling, the ubiquitination regulatory pathway did participate in the resistance to freezing in seeds. The expression of ubiquitination-related genes and ICE1 gene were detected by real-time quantitative PCR. It was found that Parthenolide inhibited the expression of RING-finger E3 ligase COP1 and anti-freezing factor ICE1 during the slow cooling. The effects of Parthenolide on the activities of SOD were further proved that during the programmed cooling process, Parthenolide may destroy the freezing resistance system of lettuce seeds.
Pages: 308-314
Year: 2016 Issue: 4
Journal: Subtropical Plant Science
Keyword: Lactuca sativa; seed; programmed cooling; cooling rate; chilling stress; Parthenolide;
Abstract: In order to study the effects of Parthenolide on chilling resistance of Lactuca sativa seeds during programmed cooling, the seeds were imbibed by exogenous animal ubiquitin E3 ligase activator Parthenolide before treated with programmed cooling with different cooling rates. The results showed that the addition of Parthenolide reduced the freezing tolerance of lettuce seeds in both fast and slow cooling treatments, and the germination rates after both cooling treatments were significantly lower than control, the effects of Parthenolide were the most obvious when the temperature was reduced to -20 ℃, which indicating that during the programmed cooling, the ubiquitination regulatory pathway did participate in the resistance to freezing in seeds. The expression of ubiquitination-related genes and ICE1 gene were detected by real-time quantitative PCR. It was found that Parthenolide inhibited the expression of RING-finger E3 ligase COP1 and anti-freezing factor ICE1 during the slow cooling. The effects of Parthenolide on the activities of SOD were further proved that during the programmed cooling process, Parthenolide may destroy the freezing resistance system of lettuce seeds.
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