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Effect of hepatic stellate cell on epithelial-mesenchymal transition in hepatocellular carcinoma MHCC-LM3 cell line
Author(s): Li Bo, Ma Jun, He Huan, Wang Liping, Xu Ming
Pages: 809-
814
Year: 2016
Issue:
12
Journal: New Chinese Medicine
Keyword: Hepatocellular carcinoma; Hepatic stellate cell; Epithelial-mesenchymal transition;
Abstract: Objective To investigate the effect of hepatic stellate cells on the epithelial-mesenchymal transition (EMT)of hepatocellular carcinoma MHCC-LM3 cell line. Methods MHCC-LM3 cells were co-in-cubated with the culture supernatant of LX-2 in the study group,and the untreated MHCC-LM3 cells were as the control. The morphological changes,invasion ability,and the changes in the expression of E-cadherin and Vimentin proteins were statistically compared between two groups. Results Compared with the control group, the MHCC-LM3 cells co-incubated with the supernatant of LX-2 were elongated and the cell-cell junction be-came looser. Transwell assay revealed that the quantity of invasive MHCC-LM3 cells co-incubated for 12-,24-and 48-h in the study group was significantly higher compared with that in the control group (all P<0.01 ).Meantime,the quantity of invasive MHCC-LM3 cells in the study group was significantly enhanced over the time of incubation (all P<0.01). In the study group,the expression levels of E-cadherin protein after 24-and 48-h incubation were considerably lower than those in the control group (both P<0.01). The expression levels of Vimentin protein after 12-,24- and 48- incubation in the study group were significantly up-regulated com-pared with those in the control group (all P<0.01). Conclusion Hepatic stellate cells can promote the EMT and enhance the invasion of hepatocellular carcinoma MHCC-LM3 cell line.
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