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Intervention of systolic pressure and left ventricular hypertrophy in rats under cold stress
Author(s): SUN Chuanfu, WANG Sugui, PENG Yigen, SHI Yan, DU Yeping, SHI Guoxiang, WEN Tong, WANG Yunkai, SU Hai
Pages: 438-
443
Year: 2016
Issue:
6
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword: Chills; Stress; Systolic pressure; Left ventricular hypertrophy;
Abstract: Objective To investigate the effects of different drugs on systolic blood pressure (SBP) and left ventricular hypertrophy (LVH) in spontaneously hypertensive rats under cold stress.Methods A total of 40 male spontaneously hypertensive rats aged 10 weeks (160~200 g) were given adaptive feeding for 7 days at a temperature of 20±1°C and then randomly divided into control group,cold stress group,metoprolol group,amlodipine group,and benazepril group,with 8 rats in each group.SBP,body weight,and heart rate were measured once a week.After the rats were sacrificed by exsanguination,left ventricular weight (LVW) was measured,and left ventricular weight index (LVWI;mg/g) was calculated.Radioimmunoassay was used to measure the concentrations of endothelin-1 (ET-1) and angiotensin-Ⅱ (Ang-Ⅱ) in plasma and myocardium,and the chemical method was used to measure the concentrations of nitric oxide (NO) in plasma and myocardium.RT-PCR was used to measure the mRNA expression of endothelin-A receptor.Results Compared with the cold stress group,all medication groups showed significant reductions in SBP since week 5 (P<0.05).The cold stress group showed a significant increase in LVWI compared with the control group (3.38±).27 mg/g vs 2.89±0.19 mg/g,P<0.05).The amlodipine group showed a significant reduction in LVWI compared with the cold stress group (2.98±0.28 mg/g vs 3.38±0.27 mg/g,P<0.05).The cold stress group showed a significant reduction in plasma NO concentration compared with the control group (104.9±19.5 μmol/L vs 129.3 ±17.8 μmol/L,P<0.05);compared with the cold stress group,all the medication groups showed significant increases in blood NO concentration (P<0.05).The cold stress group showed a significant increase in myocardial ET-1 concentration compared with the control group (6.3±1.5 pg/100 mg vs 4.5±1.9 pg/100 mg,P<0.05);compared with the cold stress group,the amlodipine group showed a significant reduction in myocardialET-1 concentration (4.4±1.0 pg/100mg vs 6.3 ±1.5 pg/100 mg,P<0.05).The cold stress group had significantly higher mRNA expression of endothelin-A receptor than the control group (0.86±0.23 vs 0.45±0.16,P<0.01);compared with the cold stress group,the amlodipine group showed a significant reduction in the mRNA expression of endothelin-A receptor (0.41±0.14 vs 0.86±0.23,P<0.01).Conclusion Amlodipine can reduce the increase in SBP and inhibit LVH in spontaneously hypertensive rats under cold stress.
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