The server is under maintenance between 08:00 to 12:00 (GMT+08:00), and please visit later.
We apologize for any inconvenience caused
Login  | Sign Up  |  Oriprobe Inc. Feed
China/Asia On Demand
Journal Articles
Laws/Policies/Regulations
Companies/Products
Protective effect of Nrf2 activation by curcumin against lead-induced toxicity and apoptosis in SH-SY5Y cells
Author(s): 
Pages: 401-405
Year: Issue:  6
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  Lead acetateSH-SY5Y cellApoptosisCurcuminNuclear factor erythroid 2-related factor 2;
Abstract: Objective To study the protective effect of nuclear factor erythroid 2-related factor 2 (Nrf2)activation by curcumin against lead-induced toxicity and apoptosis in SH-SY5Y human neuroblastoma cells and its impact on expression of apoptosis-related proteins.Methods After the cells were treated with 0,0.5,1,5,or 10 μmol/L curcumin for 24 hours,nucleoprotein was extracted and electrophoretic mobility shift assay was used to measure Nrf2-antioxidant responsive element (ARE) binding capacity.The optimal concentration of curcumin was figured out for treating cells.After pretreatment with 5 μmol/L curcumin for 24 hours,cells were exposed to lead acetate at different concentrations (0,5,25,and 125 μmol/L for control,low-dose,mediumdose,and high-dose groups).The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay,flow cytometry,and Western blot were used to evaluate cell viability,apoptosis,and expression of apoptosisrelated proteins,respectively.Results Curcumin of 5 μmol/L caused significantly increased cell viability in low-,medium-,and high-dose groups exposed to lead acetate for 12 hours(98.42%±1.12% vs 92.92%±0.14%,P<0.05;95.30%±1.17% vs 91.15%±0.67%,P<0.05;94.50%±1.45% vs 85.98%±0.45%,P<0.05).Curcumin of 5 μmol/L also caused significantly increased cell viability in medium-and high-dose groups exposed to lead acetate for 24 hours (93.10%±1.63% vs 88.40%±4.13%,P<0.05;90.13%±2.03% vs 83.63%±3.42%,P<0.05).The high-dose group had a significantly higher apoptotic rate than the control group 6.17% ±1.31% vs 3.30%±0.53%,P<0.05).Curcumin of 5.0 μmol/L significantly reduced the apoptotic rate in the high-dose group (2.97%±0.15% vs 6.17%±1.31%,P<0.05).Exposure to lead acetate elevated the expression of Bax,cytochrome C,and caspase-3 and reduced Bcl-2 expression.Curcumin of 5.0 μmol/L significantly reduced the expression of Bax,cytochrome C,and caspase-3 in the high-dose group (P<0.05).Conclusion Nrf2 activation by curcumin has a protective effect against lead-induced toxicity and apoptosis in SH-SY5Y cells.The protective effect of Nrf2 against apoptosis may be associated with the regulation of apoptosis-related proteins.
Related Articles
No related articles found