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Influence of puerarin on proliferative activity of human fetal scleral fibroblasts and expression of collagen type Ⅰ and matrix metalloproteinase-2 in power frequency electromagnetic field
Author(s): Su Qiang, Yang Lu
Pages: 447-
451
Year: 2016
Issue:
6
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword: Puerarin; Electromagnetic fields; Human fetal scleral fibroblast; Collagen type Ⅰ; Matrix metalloproteinase-2;
Abstract: Objective To investigate the influence of puerarin on the proliferative activity of human fetal scleral fibroblasts (HFSFs) and the expression of collagen type Ⅰ and matrix metalloproteinase-2 (MMP-2) in HFSFs in the power frequency electromagnetic field.Methods HFSFs were cultured and divided into four radiation groups (0.2 mT,50 Hz) and control group.Puerarin at concentrations of 0.0,1.0,5.0,and 10.0 μmol/L was added to the four radiation groups,respectively.The methyl thiazolyl tetrazolium colorimetry was used to measure the influence on the proliferative activity of HFSFs,Western blot and RT-qPCR were used to measure the protein and mRNA expression of collagen type Ⅰ and MMP-2 induced by puerarin in the power frequency electromagnetic field.Results The radiation groups and control group showed significant increases in the proliferative activity of HFSFs over the culture time (F=959.472 and 279.468,both P<0.01).At 24 and 48hours,the radiation groups showed significantly lower proliferative activity than the control group (0.432±0.038/0.591±0.017 vs 0.536±0.034/0.801±0.020,both P<0.01).With the increasing concentration of puerarin (0.0,1.0,5.0,and 10.0 μmol/L),the proliferative activity of HFSFs significantly increased,with A values of 0.598±0.031,0.809±0.041,0.910±0.037,and 0.983±0.054,respectively (P<0.05).After exposure for 24 hours,the radiation groups showed significantly lower protein expression of collagen type Ⅰ and significantly higher protein expression of MMP-2 (t=7.917 and 7.831,both P<0.01);compared with the 0.0 μmol/L puerarin group,the 1.0,5.0,and 10.0 μmol/L puerarin groups showed significant increases in the protein expression of collagen type Ⅰ and significant reductions in the protein expression of MMP-2 (all P<0.01).Compared with the control group,the radiation groups showed significant reductions in the mRNA expression of collagen type Ⅰ and MMP-2 (t=17.293and 16.378,both P<0.01);compared with the 0.0 μmol/L puerarin group,the 1.0,5.0,and 10.0 μmol/L puerarin groups showed significant increases in the mRNA expression of collagen type Ⅰ and significant reductions in the mRNA expression of MMP-2(P<0.01).Conclusion Puerarin can inhibit the reduction in the proliferative activity of HFSFs in the power frequency electromagnetic field,upregulate the expression of collagen type Ⅰ,downregulate the expression of MMP-2,and thus exert its protective effect.
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