Author(s): Zhang Zhaoqiang, Zhang Chunzhi, Nie Jichi, Lin Li
Pages: 443-446
Year: 2016 Issue:  6
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword:  Vibration; Rabbits;
Abstract: Objective To study the effects of expression of calpain mRNA in rabbits exposed to vibration by hind legs.Methods 32 New Zealand rabbits were randomly divided into a control group and 3 experimental groups according to 4-hour energy-equivalent frequency-weighted acceleration[a()(4)]:low (4.33 m/s2),moderate (8.67 m/s2) and high (17.34 m/s2) intensity group to accepted the vibration by hind legs.45 ds later,brain and skeletal muscle tissue of rabbits were taken to detect the expression of calpain-1 and calpain-2 mRNA by RT-qPCR technique.Results The relative content of calpain-1 mRNA in the brain tissues in rabbits of low,medium and high intensity group were 8.35±3.75,9.64±4.54,5.10±5.26.While the relative content of calpain-2 mRNA in the brain tissues in rabbits of low,medium and high intensity group were 7.34 ±4.97,8.50 ±5.66,8.16 ±5.59.Compared with the control group(1.10±0.29,0.56±0.43),the expression of calpain-1 and calpain-2 mRNA of the intensity groups showed an significantly increasing trend(P＜0.01).In skeletal muscle tissue,the relative content of calpain-1 mRNA were 4.36±2.05,7.37±4.06,12.46±6.21.Compared with the control group (0.98±0.59),the expression of calpain-1 mRNA of experiment groups were significantly higher (P＜0.05).The expression of calpain-2 mRNA of the intensity groups had no significantly difference with the control group (P＞0.05).Conclusion The expression of calpain-1 and calpain-2 mRNA can be promoted by the vibration by the hind legs.