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Determination of HVA and VMA in urine using high performance liquid chromatography-tandem mass spectrometry
Author(s): 
Pages: 461-465
Year: Issue:  6
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  Chromatographyhigh pressure liquidUrineHomovanillic acidVanil mandelic acid;
Abstract: Objective To establish a method for the determination of homovanillic acid (HVA) and vanil mandelic acid (VMA) in urine,using high performance liquid chromatography-tandem mass spectrometry (HPLC/MS/MS)detection.Methods A 200 μl volume of urine sample added with 20 μl chlorzoxazone internal standard was mixed in a centrifuge tube,then added 100μL methanol (containing 1% acetic acid),vortex mixed and centrifuged at 13 000 rpm for 1 min.A 10 μl volume of the above supernatant solution was injected into HPLC/MS/MS system for quantitative analysis.Results With internal standard method,the calibration curves of HVA and VMA were linear in the range of 20~10 000 ng/ml and 20 ~7 500 ng/ml,with correlation coefficient of 0.995 6 and 0.997 0,respectively.The LOQ of HVA and VMA was 4.69 ng/ml and 2.33 ng/ml,respectively.The method recoveries were in the range of 85.72%~117.95% for HVA,and 95.82%~113.78% for VMA.Conclusion This method is simple,rapid and characterized with acceptable sensitivity and accuracy to meet the requirements for the analysis of homovanillic acid and vanilla mandelic acid.
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