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Prokaryotic Expression of Procalcitonin and Preparation of Anti-procalcitonin Monoclonal Antibody
Pages: 147-155
Year: Issue:  2
Journal: Acta Laser Biology Sinica

Keyword:  procalcitoninProkaryotic Expressionenzyme-linked immunosorbent assay (ELISA )purificationmonoclonal antibody (mAb);
Abstract: Purpose:Recombinant human PCT was efficiently expressed in E.coli,and was purified,and then the high-ly sensitive and specific monoclonal antibodies (mAb)against PCT were prepared,which can be used as diagnostic raw materials for infectious diseases.Method:The recombinant PCT was purified with saturated ammonium sulfate precipita-tion and affinity chromatography method,and then the recombinant protein was confirmed by mass spectrometry.West-ern blot and indirect ELISA assay was used to detect the immunoreactivity of the recombinant protein.The mice were im-munized with the recombinant protein,and anti-PCT monoclonal antibodies (mAb)were prepared by cell fusion andscreening.Result:The recombinant protein was the human procalcitonin,which had good immunoreacitvity and immu-nogenicity,and 7 monoclonal antibodies against human PCT were obtained,and ELISA indicated the antibodies had a good specificity and sensitivity to PCT antigen.Conclusion:Highly sensitive and specific anti-PCT monoclonal antibody was prepared for recombinant human PCT,which will provide a raw material for the development of PCT rapid diagnostic reagent.
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