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Inhibitory Effect of AcSDKP on the Myofibroblast Differentiation of Rat Mesenchymal Stem Cells Induced by Transforming Growth Factor-β1 in vitro
Author(s): 
Pages: 142-146,184
Year: Issue:  2
Journal: Acta Laser Biology Sinica

Keyword:  MSCsAcSDKPTGF-β1fibrosis;
Abstract: Objective:To investigate the effects of antifibrotic peptide N-acetyl- seryl-aspartly-lysyl-proline (AcSDKP) on the differentiation of rat MSCs into myofibroblasts mediated by transforming growth factor beta- 1 (TGF-β1 ).To ex-plore the possible anti-fibrotic mechanisms of AcSDKP in depth. Methods:Isolated the mesenchymal stem cells (MSCs)by the whole bone marrow culture method.The protein expressions of α-SMA and the changes of phospho-Smad2/3 and phospho-ERK1/2 were detected by immunocytochemistry and Western blotting.Results:Compared with the control group,the protein expressions ofα-SMA,p-Smad2/3,p-ERK1/2 were greatly increased in MSCs which was induced by TGF-β1 ,and showed a dose-dependent decrease in AcSDKP group.Conclusion:AcSDKP markedly inhibit the differentiation of rat MSCs into myofibroblasts probably by blocking the TGF-β/Smad/ERK1/2 signaling pathway ac-tivation,and thus mediating its anti fibrosis activity.
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