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Effect of remote ischemic pre-conditioning on myocardial ischemic injury in a hemorrhagic shock-reperfusion rat model
Author(s): Lin Hui, Liu Juanhua, Zhou Tianen, Yang Zhengfei
Pages: 227-
230
Year: 2016
Issue:
4
Journal: New Chinese Medicine
Keyword: Shock; Myocardialreperfusioninjury; Ischemicpre-conditioning; Cardiacfunction; Troponin I;
Abstract: Objective Cardiacultrasound,electrocardiogramandmeasurementofmyocardialinjury markers were performed to comprehensively investigate the effect of remote ischemic pre-conditioning (RIPC) uponmyocardialischemicinjuryinahemorrhagicshock-reperfusionratmodel.Methods Thirtysixmale adult Sprague-Dawley rats,weighing 300-350 g,were randomly divided into the sham control (C),hemorrhag-ic shock-reperfusion (SR)and RIPC groups (n=1 2 for each group). Hemorrhagic shock-reperfusion rat mod-els were established by 60-min bleeding (50% of total blood volume)via femoral artery followed by 30-min reperfusion via femoral vein at 1 h later. In the RIPC group,bilateral hind limbs were clamped at 40 min before hemorrhage. The procedure involved four cycles of 5 min of ischemia followed by 5 min of reperfusion. Electro-cardiographic tracing and mean arterial pressure (MAP)were continuously recorded for at baseline (BL),im-mediately after bleeding (S1 ),before reperfusion (S2)and after reperfusion (R1 )and 1 h after reperfusion (PR1 h)and 2 h after reperfusion (PR2h,respectively. Cardiac output (CO),left ventricular ejection fraction (LVEF),fractional shortening (FS)and Tei Index were measured by electrocardiogram. The concentration of cardiac troponin I (cTnI)in the arterial blood was measured at BL,S2 and PR2h. The survival of animals was observedat72afterresuscitation.Results ComparedwiththeCgroup,theMAP,COandFSintheSRgroup at S1-PR2h,and in the RIPC group at S1-S2 were significantly decreased whereas Tei Index was considerably elevated (all P<0.01 ). Compared with the SR group,the MAP,CO and FS were significantly decreased whereas Tei Index was considerably increased in the RIPC group after resuscitation (all P<0.01 ). From S1 to PR2h,ST-segment depression and cTn I concentration in the SR and RIPC groups were significantly higher than those in the C group (all P<0.01 ),whereas the degree of increase in the RIPC group was significantly less than that in the SR group (P<0.01 ). The survival rate after resuscitation in the RIPC group was also sig-nificantlyhighercomparedwiththatintheSRgroup(P<0.01).Conclusion RIPCeffectivelyalleviatesmy-ocardial ischemic injury and significantly enhances prognosis outcomes in a hemorrhagic shock-reperfusion rat model.
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