Rapid propagation in vitro and rooting characteristics of blueberry
Author(s): HUANG Ke, HUANG Qin, DAI Huan, LIU Yi-qing, ZOU Yong, Forestry and Life Sciences College, Chongqing University of Arts and Science, Research Institute of Special Plant, Chongqing University of Arts and Science, Chongqing Engineering Research Center for Special Plant Seedlings, Chongqing Garden Flower Engineering Research Center
Pages: 140-146
Year: 2016 Issue: 1
Journal: Nonwood Forest Research
Keyword: blueberry; tissue culture; rooting; endogenous hormone;
Abstract: In order to research propagation techniques in vitro and rooting characteristics of blueberry, taking spring stem segments of blueberry ‘Sharpblue’ as explants, projects of primary inducing, proliferation culture, and rooting culture were screened, and calli in the base of new shoot were observed through section, and the concentrations of endogenous IAA and ZR in new buds were determined on the 0, 2nd, 4th, 6th day of rooting period. The results showed that the optimal inducing medium was CQWL + 3 mg·L- 1 ZT, and induction rate could reach 45.22%. The optimal proliferation medium was CQWL + 1.5 mg·L- 1 ZT, proliferation coefficient was over 4.2 times, and the adventitious buds were healthy and strong. The optimal rooting medium was 1/2 CQWL + 0.05 mg·L- 1 IBA, a plant had 4-5 roots, and rooting rate reached 73%. The results of section observation showed that the cells in calli of new shoots base with roots was smaller and had more cytoplasm. In the 0.05 mg·L- 1 IBA treatment, the concentration of endogenous IAA in new shoots was increased but not significant during rooting, the concentration of endogenous ZR was decreased and then increased, and the rate of IAA to ZR was stable. Thus CQWL medium was suitable for tissue culture of blueberry, ZT could promote induction and proliferation, and IBA could promote blueberry rooting in tissue culture.
Pages: 140-146
Year: 2016 Issue: 1
Journal: Nonwood Forest Research
Keyword: blueberry; tissue culture; rooting; endogenous hormone;
Abstract: In order to research propagation techniques in vitro and rooting characteristics of blueberry, taking spring stem segments of blueberry ‘Sharpblue’ as explants, projects of primary inducing, proliferation culture, and rooting culture were screened, and calli in the base of new shoot were observed through section, and the concentrations of endogenous IAA and ZR in new buds were determined on the 0, 2nd, 4th, 6th day of rooting period. The results showed that the optimal inducing medium was CQWL + 3 mg·L- 1 ZT, and induction rate could reach 45.22%. The optimal proliferation medium was CQWL + 1.5 mg·L- 1 ZT, proliferation coefficient was over 4.2 times, and the adventitious buds were healthy and strong. The optimal rooting medium was 1/2 CQWL + 0.05 mg·L- 1 IBA, a plant had 4-5 roots, and rooting rate reached 73%. The results of section observation showed that the cells in calli of new shoots base with roots was smaller and had more cytoplasm. In the 0.05 mg·L- 1 IBA treatment, the concentration of endogenous IAA in new shoots was increased but not significant during rooting, the concentration of endogenous ZR was decreased and then increased, and the rate of IAA to ZR was stable. Thus CQWL medium was suitable for tissue culture of blueberry, ZT could promote induction and proliferation, and IBA could promote blueberry rooting in tissue culture.
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