Establishment and Assessment of the Cut- off Values of Anti- cyclic Citrullinated Peptide Antibody,Rheumatoid Factor and C- reactive Protein in Serum for Diagnosis of Rheumatoid Arthritis
Author(s): Liu Weiping, He Limei, Yin Minggang, Department of Clinical Laboratory, Zigong First People’s Hospital
Pages: 131-134
Year: 2016 Issue: 2
Journal: Journal of Medical Research
Keyword: Rheumatoid arthritis; Anti-cyclic citrullinated peptide antibody; Rheumatoid factor; C-reactive protein;
Abstract: Objective To establish and assess the cut- off values of anti- cyclic citrullinated peptide antibody( anti- CCP),rheumatoid factor( RF) and C- reactive protein( CRP) in serum for rheumatoid arthritis( RA). Methods A total of 130 RA,130 non- RA patients and 100 healthy people from June 2014 to March 2015 were enrolled. The concentration of anti- CCP was analyzed by ELISA,and the concentration of RF and CRP were analyzed by immunity transmission turbidity. The optimal diagnostic cut- off values of anti-CCP,RF and CRP for RA were determined by ROC,and the diagnostic performance of them were assessed. Results The concentration of RF in RA and non- RA group were 275. 9 ± 317. 6IU / ml and 42. 8 ± 131. 3IU / ml respectively and the former was significantly higher than the latter.( t = 7. 571,P < 0. 05). The concentration of CRP in the RA and non RA group were 39. 2 ± 47. 3mg / L and 29. 7 ±42. 2mg / L respectively and the former was significantly higher than the latter( t = 2. 602,P = 0. 015). The area under ROC curve( AUC)of anti- CCP,RF and CRP were 0. 868( 0. 820- 0. 916),0. 785( 0. 732- 0. 846),0. 587( 0. 512- 0. 661),respectively. For 10 RU /ml as cut- off value of anti- CCP,the sensitivity,specificity and Youden index was 0. 669,0. 892 and 0. 562,respectively. For 25 IU /ml as cut- off value of RF,sensitivity,specificity and Youden index were 0. 715,0. 777 and 0. 492,respectively. For 30 mg / L as cut-off value of CRP,sensitivity,specificity and Youden index were 0. 469,0. 623 and 0. 092,respectively. Conclusion The optimal diagnosis cut- off values of anti- CCP,RF and CRP were 10 RU / ml,25 IU / ml,and 30 mg / L,respectively. The diagnostic performance of anti- CCP was higher than that of RF,which was better than that of CRP.
Pages: 131-134
Year: 2016 Issue: 2
Journal: Journal of Medical Research
Keyword: Rheumatoid arthritis; Anti-cyclic citrullinated peptide antibody; Rheumatoid factor; C-reactive protein;
Abstract: Objective To establish and assess the cut- off values of anti- cyclic citrullinated peptide antibody( anti- CCP),rheumatoid factor( RF) and C- reactive protein( CRP) in serum for rheumatoid arthritis( RA). Methods A total of 130 RA,130 non- RA patients and 100 healthy people from June 2014 to March 2015 were enrolled. The concentration of anti- CCP was analyzed by ELISA,and the concentration of RF and CRP were analyzed by immunity transmission turbidity. The optimal diagnostic cut- off values of anti-CCP,RF and CRP for RA were determined by ROC,and the diagnostic performance of them were assessed. Results The concentration of RF in RA and non- RA group were 275. 9 ± 317. 6IU / ml and 42. 8 ± 131. 3IU / ml respectively and the former was significantly higher than the latter.( t = 7. 571,P < 0. 05). The concentration of CRP in the RA and non RA group were 39. 2 ± 47. 3mg / L and 29. 7 ±42. 2mg / L respectively and the former was significantly higher than the latter( t = 2. 602,P = 0. 015). The area under ROC curve( AUC)of anti- CCP,RF and CRP were 0. 868( 0. 820- 0. 916),0. 785( 0. 732- 0. 846),0. 587( 0. 512- 0. 661),respectively. For 10 RU /ml as cut- off value of anti- CCP,the sensitivity,specificity and Youden index was 0. 669,0. 892 and 0. 562,respectively. For 25 IU /ml as cut- off value of RF,sensitivity,specificity and Youden index were 0. 715,0. 777 and 0. 492,respectively. For 30 mg / L as cut-off value of CRP,sensitivity,specificity and Youden index were 0. 469,0. 623 and 0. 092,respectively. Conclusion The optimal diagnosis cut- off values of anti- CCP,RF and CRP were 10 RU / ml,25 IU / ml,and 30 mg / L,respectively. The diagnostic performance of anti- CCP was higher than that of RF,which was better than that of CRP.
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