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gui mo mu chang niu nai zhong da fu sha xing can liu jian ce fang fa de yan jiu
Author(s): WU Erbin, LI Qiang, LI Lin
Pages: 24-
26
Year: 2015
Issue:
19
Journal: China Dairy Cattle
Keyword: 牛奶; 达氟沙星; 残留; 检测; 间接竞争ELISA;
Abstract: 本研究选择乳中β-羟基丁酸的测定值小于1mmol/L的奶牛个体,以达氟沙星(DFLX)为对象,以先期制备的兔源达氟沙星抗体和包被原为基础,建立间接竞争ELISA检测方法。该方法的最低检测量为27.29ng/mL,回归方程为y=-18.776x+93.634(R2=0.9918),在0.5~2000ng/mL范围内样品添加平均回收率为91.47%。结果表明,该方法可用于乳中β-羟基丁酸的测定值小于1mmol/L的奶牛个体所产牛奶中达氟沙星药物残留的筛选检测。
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