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Clinical analysis of chromosome karyotype and single nucleotide polymorphism array in prognostic e-valuation of acute myeloid leukemia
Author(s): Dai Qiangsheng, Chen Zhiyong, Zhang Bo, Long Jianting, Li He-ping
Pages: 737-
742
Year: 2015
Issue:
11
Journal: New Chinese Medicine
Keyword: Acute myeloid leukemia; Single nucleotide polymorphism; Karyotyping; Loss of heterozygosity;
Abstract: Objective To analyze G-banding karyotyping and single nucleotide polymorphism array (SNPa)method in evaluation of prognosis of acute myeloid leukemia (AML),especially for those with normal chromosome karyotype.Methods G-banding karyotyping and SNPa analysis of the bone marrow from 23 AML patients were performed to detect the chromosome karyotype and SNP status.Clinical prognosis of those patients was observed and the relationship between clinical prognosis and alterations in chromosome genetic information was evaluated.Results Among 23 AML patients detected by G-banding karyotyping,11 were accompanied with abnormal chromosome karyotype and 12 were normal.At the same time,SNPa analysis revealed that 19 patients showed abnormal genetic information and four were normal.Eleven of the twelve patients with normal chromosome karyotype were accompanied by SNP,four of whom presented with loss of heterozygosity (LOH). Among 12 patients with normal chromosome karyotype,seven gained complete remission after the first chemo-therapy induction,and still survived until now.Another two obtained complete remission following the second chemotherapy induction.Three patients failed to obtain complete remission after the first chemotherapy induc-tion and died of severe infection or hemorrhage after the second chemotherapy induction.Among 11 patients with abnormal chromosome karyotype,eight obtained complete remission after the first chemotherapy induction. SNPa indicated that four patients with LOH failed to gain complete remission,probably caused by the occur-rence of SNP in the specific sites of long arm of chromosome 17 or 21.Conclusion SNPa can serve as a com-plimentary tool in assessing the prognosis of AML,especially for those with normal chromosome karyotype.
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