Identification and Cloning of Tillering-Related GenesOsMAX1 in Rice
Author(s): WANG Xiu-mei, LIANG Yue-yang, LI Ling, GONG Chang-wei, WANG Hai-peng, HUANG Xiao-xi, LIShuang-cheng, DENG Qi-ming, ZHU Jun, ZHENG Ai-ping, LI Ping, WANG Shi-quan
Pages: 255-263
Year: 2015 Issue: 6
Journal: Rice Science
Keyword: rice; strigolactone; OsMAX1; gene cloning; tillering; phytohormone;
Abstract: Tillering is an important agronomic trait which has a direct impact on plant type and grain yield. Strigolactones are a class of important phytohormones regulating rice tillering.ATMAX1 is an important gene involved in strigolactone biosynthesis through encoding the protein P450 inArabidopsis. Based on sequence BLASTp, we identified five homologous genes ofATMAX1in rice, i.e.,OsMAX1a,OsMAX1b, OsMAX1c,OsMAX1d andOsMAX1e. Among them,OsMAX1a andOsMAX1e showed stable and high expression in rice tissues. In addition, we observed thatOsMAX1a andOsMAX1e can rescue the branched phenotype and the influences caused byMAX1 mutation inArabidopsis. Moreover, the expression of OsMAX1a andOsMAX1e can respond to phosphate deficiency and different phytohormones, especially GR24, a strigolactone analogue. Therefore, it is concluded thatOsMAX1a andOsMAX1e are involved in the biosynthesis of strigolactones and regulated rice tillering.
Pages: 255-263
Year: 2015 Issue: 6
Journal: Rice Science
Keyword: rice; strigolactone; OsMAX1; gene cloning; tillering; phytohormone;
Abstract: Tillering is an important agronomic trait which has a direct impact on plant type and grain yield. Strigolactones are a class of important phytohormones regulating rice tillering.ATMAX1 is an important gene involved in strigolactone biosynthesis through encoding the protein P450 inArabidopsis. Based on sequence BLASTp, we identified five homologous genes ofATMAX1in rice, i.e.,OsMAX1a,OsMAX1b, OsMAX1c,OsMAX1d andOsMAX1e. Among them,OsMAX1a andOsMAX1e showed stable and high expression in rice tissues. In addition, we observed thatOsMAX1a andOsMAX1e can rescue the branched phenotype and the influences caused byMAX1 mutation inArabidopsis. Moreover, the expression of OsMAX1a andOsMAX1e can respond to phosphate deficiency and different phytohormones, especially GR24, a strigolactone analogue. Therefore, it is concluded thatOsMAX1a andOsMAX1e are involved in the biosynthesis of strigolactones and regulated rice tillering.
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