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Association between FOXP3 promoter methylation and the expression of FOXP3 protein in unexplained ;recurrent spontaneous abortion
Author(s): Hou Wenhui, Li Yinguang, Li Zhuyu, Li Jie, Fang Liyuan, Li Xiao-qing, You Zeshan
Pages: 580-
583
Year: 2015
Issue:
9
Journal: New Chinese Medicine
Keyword: Unexplained recurrent spontaneous abortion; FOXP3; Promoter; Methylation;
Abstract: Objective To investigate the association between the level of FOXP3 promoter methyla-tion and the expression level of FOXP3 protein in the decidua of patients with unexplained recurrent spontane-ous abortion (URSA).Methods The expression levels of FOXP3 protein in the decidua of 20 URSA patients and 20 healthy counterparts who underwent induced abortion were assessed by Western blot.Bisulfite-assisted genomic sequencing PCR (BSP)was utilized to detect the levels of FOXP3 promoter methylation.The correla-tion between two indexes was evaluated.Results The expression level of FOXP3 in the decidua of the URSA group was significantly lower than that of the control group (P <0.01),whereas the level of FOXP3 promoter methylation in the URSA group was significantly higher compared with that in the control group (P <0.01).The expression level of FOXP3 protein was negatively correlated with the level of FOXP3 promoter methylation (r =-0.917,P <0.01).Conclusion As the level of FOXP3 promoter methylation increases,the expres-sion level of FOXP3 protein is down-regulated,which may cause abnormal immune tolerance and potentially lead to the incidence of RSA.
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