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Comparative study of cell culture, fluorescent PCR and LCR in detection of C. trachomatis
Author(s): 
Pages: 146-148
Year: Issue:  3
Journal: JOURNAL OF CLINICAL DERMATOLOGY

Keyword:  沙眼衣原体泌尿生殖道荧光PCRLCR细胞培养;
Abstract: 为了比较细胞培养、连接酶链反应(LCR)和荧光PCR技术在检测性传播疾病门诊患者标本沙眼衣原体的意义.分别在国内5家临床医院性传播疾病门诊收集到673份尿道/宫颈拭子标本,进行沙眼衣原体培养和荧光PCR检测,检测结果不相符合的标本采用LCR复检,比较分析PCR与培养、LCR以及综合结果的相符性.结果合格病例616例,培养和荧光PCR的检测阳性率分别为6.33%和27.1%,与培养相比,荧光PCR法的敏感性为100%.LCR复核标本200份,与之相比,荧光PCR的敏感性为98.6%,特异性89.5%,YI指数为0.881.综合分析证明荧光PCR检测沙眼衣原体的敏感性为98.78%,特异性为98.67%,YI指数0.9745.结合临床表现发现,男性患者出现尿道炎症体征意义大于症状,而在女性患者症状或体征均不特异.结果表明国产荧光PCR技术检测尿道/宫颈拭子沙眼衣原体具有较高的敏感性与特异性,可以用于临床检验,实验室质控与监督是本方法得以正确应用的关键.
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