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Studies of Biological Effects of Static Magnetic Fields on Vitro Catalase from Bovine Liver in Two Different Conformational States
Pages: 98-103
Year: Issue:  2

Keyword:  catalasestatic magnetic fieldsconformational statesbiological effects;
Abstract: Biological effects of 0.23~0.61T static magnetic fields on vitro catalase from bovine liver with two different conformation were studied in this paper by way of activity assay, analyses of ultraviolet differential spectra, multiple dimension fluorescence spectra, and differential scanning calorimetric analyses. The selected conformationals were 4?℃ inhibitive and 25?℃ active states. The enzyme of 4?℃ have different conformation from that of 25?℃. After allowed to stand under 0.23~0.61T magnetic fields for various periods of time, as far as the enzymatic solution of 4?℃ was concerned, in which temperature the enzymatic molecules were in inhibitive state, the enzyme showed little biological effects judging by activity assay and spectrum analyses; by contrast, when the temperature of the natural enzymatic solution was 25?℃, in which temperature the enzyme molecule were activated, after it was exposed in various intensities of magnetic fields for various periods of time the enzyme showed distinct biological effects: its activities rose, and conformation also changed as well. Conformational changes resulted in wavelength λ210~310?nm ultraviolet differential spectra, fluctuations of fluorescence intensity at λ330?nm emission peak, increment of fluorescence polarization, and emersion of differential scanning calorimetric curves. Different effects of magnetic fields on enzyme between 4?℃ inhibitive and 25?℃ active states may be essentially caused by the difference of their initial conformational states when they were treated in the same setting.
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