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Study on the Autofluorescence in Fungi with Laser Scanning Confocal Microscope
Author(s): 
Pages: 109-113
Year: Issue:  2
Journal: ACTA LASER BIOLOGY SINICA

Keyword:  真菌自发荧光激光共聚焦显微镜;
Abstract: 本试验用激光共聚焦扫描显微镜对三株不同真菌的菌丝、分生孢子以及子实体的自发荧光现象进行了观察.结果发现:处于相同培养条件的三株真菌的荧光分布特点不一,并且同一真菌在不同培养基条件下所表现的荧光现象亦有差异,说明真菌的自发荧光具有种属特导性并受到培养基质等因素的影响;而培养时间对荧光强度及类别的影响不显著.化学试剂的处理试验表明,弱碱性物质对真菌的荧光具有减弱效应,而强碱性物质对真菌的荧光则表现为增强,提示不同真菌细胞内的自发荧光物质结构上和组分上的差异,其变化可导致荧光性质的改变.
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