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Raman Spectroscopic Study on EC9706 Cells Irradiated by X-ray and 60 Co γ-ray
Pages: 77-82
Year: Issue:  1
Journal: Acta Laser Biology Sinica

Keyword:  Raman spectroscopyEC9706 cellsX -ray60 Coγ-raydosage;
Abstract: After the esophagus cancer cells (EC9706)passed through the different dosages X-ray and 60 Co γbeam ex-posure and continued to cultivate 24 h,we used laser Raman spectroscopy analysis of EC9706 cell interior protein,nu-cleic acid,fats and some biological macromolecule’s conformation and content changes.The results showed that there were big differences in Raman spectrum’s intensity and frequency shift among each exposure teams and control teams. After being irradiated by X-ray,existing some bands in the control group disappeared in some dose teams’.The peak at 1 1 14 cm-1 caused by`(C-N)the skeleton chain of protein disappeared in all dose teams.After being irradiated by 60 Coγ-ray,theβfold conformation in Amide Ⅱ band changed into the random coil in the moderate dose(4,5 Gy)teams and the non-hydrogen bond degree in the di-phosphate ester (O-P-O)groups and bases enhances in big dosage teams.This provides experimental evidence to the further study on EC9706 cells’mechanism of radiation damage by X-ray and 60 Coγ-ray from the physical energy-level structure.
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