Allele Types ofRcGene of Weedy Rice from Jiangsu Province, China
Author(s): LIXiao-yan, QIANGSheng, SONGXiao-ling, CAIKun, SUNYi-na, SHIZhi-hua, DAIWei-min
Pages: 252-261
Year: 2014 Issue: 5
Journal: Rice Science
Keyword: weedy rice; red pericarp; Rcgene; allele type; origin;
Abstract: Weedy rice(Oryza sativaf.spontanea), the predominant type of which has a red pericarp, seriously inhibits growth and yield of direct-seeded rice in Jiangsu Province, China. In this study, we randomly selected 10 weedy rice accessions from 10 plots in Jiangsu, and then sequenced the full lengths of theirRcgenes (approximately 6.4 kb). In addition, we collected 166 different full-lengthRc genes in theOryzagenus from the literature and from GenBank. A collinearity sequence analysis showed that the 10 weedy riceaccessions from Jiangsu all had the same wild-type allele of theRcgene. Single nucleotide polymorphisms indicated thatthe nucleotide polymorphisms (π= 0.19) and theproportion of segregation sites(θw= 0.28) of theRcgenes in the 10 weedy rice accessions from Jiangsu were higher than those in 56 weedy rice accessions from USA (π= 0.09 andθw= 0.07). Haplotype and phylogenetic analyses showed thattheRcgenes of weedy rice accessions from Jiangsu were not revertants of therc gene found in Asian cultivated rice(O. sativa) varietieswith white pericarp. In addition,Rcgene sequences of the rice varieties Lvdao from Lianyungang, Jiangsu and Tangdao from Anhui were more similar to those of cultivated rice than to the weedy rice from Jiangsu. These findings support the continued quarantine of weedy rice and clarify the evolutionary mechanism of the red pericarp found in the weedy rice of Jiangsu.
Pages: 252-261
Year: 2014 Issue: 5
Journal: Rice Science
Keyword: weedy rice; red pericarp; Rcgene; allele type; origin;
Abstract: Weedy rice(Oryza sativaf.spontanea), the predominant type of which has a red pericarp, seriously inhibits growth and yield of direct-seeded rice in Jiangsu Province, China. In this study, we randomly selected 10 weedy rice accessions from 10 plots in Jiangsu, and then sequenced the full lengths of theirRcgenes (approximately 6.4 kb). In addition, we collected 166 different full-lengthRc genes in theOryzagenus from the literature and from GenBank. A collinearity sequence analysis showed that the 10 weedy riceaccessions from Jiangsu all had the same wild-type allele of theRcgene. Single nucleotide polymorphisms indicated thatthe nucleotide polymorphisms (π= 0.19) and theproportion of segregation sites(θw= 0.28) of theRcgenes in the 10 weedy rice accessions from Jiangsu were higher than those in 56 weedy rice accessions from USA (π= 0.09 andθw= 0.07). Haplotype and phylogenetic analyses showed thattheRcgenes of weedy rice accessions from Jiangsu were not revertants of therc gene found in Asian cultivated rice(O. sativa) varietieswith white pericarp. In addition,Rcgene sequences of the rice varieties Lvdao from Lianyungang, Jiangsu and Tangdao from Anhui were more similar to those of cultivated rice than to the weedy rice from Jiangsu. These findings support the continued quarantine of weedy rice and clarify the evolutionary mechanism of the red pericarp found in the weedy rice of Jiangsu.
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