Diversity Analysis of Intestinal Microlfora from Healthy Cow and Mastitis Cow by PCR-DGGE
Author(s): ZHAO Jie, MA Chen, XI Xiao-min, WANG Hui-yan, DING Jia, ZHANG He-ping
Pages: 1-5
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Lactobacillus; Ciliate; Intestinal microlfora; Microbiota diversity;
Abstract: In order to reveal the diversity and differences of cow with infectious mastitis and healthy cow intestinal microlfora, fecal samples of ten healthy cows and ten mastitis cows were collected in Inner Mongolia. Diversity of bacteria, Lactobacillus species and ciliate population was analyzed by denaturing gradient gel electrophoresis (DGGE). Intestinal microlfora of healthy cow and mastitis cow could be drawn with obvious differences by cluster analysis,similarity analysis and PLS-DA analysis. Compared with intestinal microflora of healthy cows, the diversity of Lactobacillus and ciliate of mastitis cows were lower. PCR-DGGE technology analyzed the diversity of intestinal microlfora and provided theoretical basis for treatment of disease by probiotic.
Pages: 1-5
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Lactobacillus; Ciliate; Intestinal microlfora; Microbiota diversity;
Abstract: In order to reveal the diversity and differences of cow with infectious mastitis and healthy cow intestinal microlfora, fecal samples of ten healthy cows and ten mastitis cows were collected in Inner Mongolia. Diversity of bacteria, Lactobacillus species and ciliate population was analyzed by denaturing gradient gel electrophoresis (DGGE). Intestinal microlfora of healthy cow and mastitis cow could be drawn with obvious differences by cluster analysis,similarity analysis and PLS-DA analysis. Compared with intestinal microflora of healthy cows, the diversity of Lactobacillus and ciliate of mastitis cows were lower. PCR-DGGE technology analyzed the diversity of intestinal microlfora and provided theoretical basis for treatment of disease by probiotic.
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