The Comparison of Microbial Diversity of Dairy Milk between Mastitis and Healthy Dairy Cows
Author(s): XI Xiao-min, MA Chen, HUANG Wei-qiang, ZHAO Jie, DING Jia, WANG Hui-yan, ZHANG He-ping
Pages: 14-20
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Bovine mastitis; Microbial community structure; Diversity;
Abstract: The PCR-DGGE fingerprinting was used to reveal the microbial community structure and bacterial species diversity of Lactobacillus and Ciliates in raw milk of mastitis and healthy dairy cows. And the result was analyzed by partial least squares-discriminant analysis. The DGGE proifles revealed that the bacterial and Ciliates’ composition of dairy milk in the mastitis cows were more complicated than those of healthy dairy cows. However, the number of bands and Shannon-Wiener index of Lactobacillus were signiifcantly lower than those of healthy dairy cows. Besides, the sequencing results indicated that the bacteria associated with mastitis cows were Streptococcus agalactiae, Escherichia coli and Uncultured Streptococcus.The bacteria associated with healthy cows were Lactobacillus and Biifdobacterium. All of those inferred that Lactobacillusin the milk may play an important role in protecting cows from being inlfected by pathogenic bacteria. Moreover, PLS-DA diagrams also clearly showed that the microbial community structure of dairy milk between mastitis and healthy dairy cows had signiifcant differences.
Pages: 14-20
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Bovine mastitis; Microbial community structure; Diversity;
Abstract: The PCR-DGGE fingerprinting was used to reveal the microbial community structure and bacterial species diversity of Lactobacillus and Ciliates in raw milk of mastitis and healthy dairy cows. And the result was analyzed by partial least squares-discriminant analysis. The DGGE proifles revealed that the bacterial and Ciliates’ composition of dairy milk in the mastitis cows were more complicated than those of healthy dairy cows. However, the number of bands and Shannon-Wiener index of Lactobacillus were signiifcantly lower than those of healthy dairy cows. Besides, the sequencing results indicated that the bacteria associated with mastitis cows were Streptococcus agalactiae, Escherichia coli and Uncultured Streptococcus.The bacteria associated with healthy cows were Lactobacillus and Biifdobacterium. All of those inferred that Lactobacillusin the milk may play an important role in protecting cows from being inlfected by pathogenic bacteria. Moreover, PLS-DA diagrams also clearly showed that the microbial community structure of dairy milk between mastitis and healthy dairy cows had signiifcant differences.
Related Articles