Isolation and Identiifcation of Lactic Acid Bacteria from Dairy Milk of Healthy Cows
Author(s): DING Jia, MA Chen, ZHAO Jie, XI Xiao-min, WANG Hui-yan, ZHNAG He-ping
Pages: 42-46
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Lactic acid bacteria; Mastitis; Holstein cow; Separation identiifcation;
Abstract: Bovine mastitis is the recurring illness of dairy farming, and it bring about huge economic losses to dairy industry. However the traditional antibiotic therapy appears many negative problems, so the lactic acid bacteria(LAB) applied to many cases of the treatment of mastitis have achieved success as the substitutes of antibiotic. Therefore, the screening of LAB which used to treatment of mastitis is particularly important. In this study, milk samples were collected from 20 head of lactation period healthy Holstein cows to isolate and screen bacteria, and use 16S rRNA gene sequences analysis to identify the isolates. As result, we isolate 22 strains of LAB that they were respectively identiifed as Lactobacillus mucosae, Enterococcus faecium, Lactococcus Lactis, Biifdo bacteriumetal. The LAB obtained from the experiment not only enriched the lactic acid bacteria species resources but also provides excellent strains of LAB resources for the future treatment of mastitis test.
Pages: 42-46
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Lactic acid bacteria; Mastitis; Holstein cow; Separation identiifcation;
Abstract: Bovine mastitis is the recurring illness of dairy farming, and it bring about huge economic losses to dairy industry. However the traditional antibiotic therapy appears many negative problems, so the lactic acid bacteria(LAB) applied to many cases of the treatment of mastitis have achieved success as the substitutes of antibiotic. Therefore, the screening of LAB which used to treatment of mastitis is particularly important. In this study, milk samples were collected from 20 head of lactation period healthy Holstein cows to isolate and screen bacteria, and use 16S rRNA gene sequences analysis to identify the isolates. As result, we isolate 22 strains of LAB that they were respectively identiifed as Lactobacillus mucosae, Enterococcus faecium, Lactococcus Lactis, Biifdo bacteriumetal. The LAB obtained from the experiment not only enriched the lactic acid bacteria species resources but also provides excellent strains of LAB resources for the future treatment of mastitis test.
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