Isolation and Identiifcation of Lactic Acid Bacteria from the Intestinal of Healthy Cows
Author(s): WANG Hui-yan, MA Chen, ZHAO Jie, XI Xiao-min, DING Jia, ZHANG He-ping
Pages: 37-41
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Lactic acid bacteria; Intestinal microbial; Healthy cows; Separation identiifcation;
Abstract: Cows feed with microbial adjuvant has many advantages in the production, therefore, live strains with good properties is of great signiifcance for production practice. Lactic acid bacteria in the gut itself has adapted to the gut enviroment, and the intestinal pathogenic bacteria have long-term antagonism competition relations with LAB, thus LAB become the best source of live strains. This experiment using the improved MRS culture medium to separate of bacteria from 20 lactation healthy Holstein cows dung, the lactic acid bacteria were isolated based on 16S rRNA gene sequences. Eventually there were 18 strains of lactic acid bacteria, lactic acid milk coccus isolated from the rumen lactobacillus. The lactic acid bacteria obtained from the experiment not only enriched the lactic acid bacteria species resources, but also made attribution to the development of lactic acid bacteria resources in China from multiple perspectives and gave a foundation for its application in animal production.
Pages: 37-41
Year: 2014 Issue: 17
Journal: China Dairy Cattle
Keyword: Lactic acid bacteria; Intestinal microbial; Healthy cows; Separation identiifcation;
Abstract: Cows feed with microbial adjuvant has many advantages in the production, therefore, live strains with good properties is of great signiifcance for production practice. Lactic acid bacteria in the gut itself has adapted to the gut enviroment, and the intestinal pathogenic bacteria have long-term antagonism competition relations with LAB, thus LAB become the best source of live strains. This experiment using the improved MRS culture medium to separate of bacteria from 20 lactation healthy Holstein cows dung, the lactic acid bacteria were isolated based on 16S rRNA gene sequences. Eventually there were 18 strains of lactic acid bacteria, lactic acid milk coccus isolated from the rumen lactobacillus. The lactic acid bacteria obtained from the experiment not only enriched the lactic acid bacteria species resources, but also made attribution to the development of lactic acid bacteria resources in China from multiple perspectives and gave a foundation for its application in animal production.
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