PRODUCTION,PURIFICATION AND CHARACTERIZATION OF RECOMBINANT CU/ZN SUPEROXIDE DISMUTASE OF BRUCELLA ABORTUS
Author(s): Shustov A V, Manat Y, Unyshiva G B, Sarina N I, Eskendirova S Z, National Center for Biotechnology
Pages: 46-51
Year: 2014 Issue: 2
Journal: Chinese Journal of Animal Infectious Diseases
Keyword: 超氧化物歧化酶; 布鲁氏菌; 体外表达; 特性分析; 流产; 表达产物; 重组; 免疫原性; 疫苗开发; 分子量;
Abstract: Immunoreactive cytosolic Cu/Zn superoxide dismutase(Cu/Zn-SOD) of Brucella abortus was expressed and characterized in the present study. The gene encoding Cu/Zn-SOD was synthesized using two-round of PCR procedure. The Cu/Zn-SOD gene was cloned into pET-22b(+) vector for expression. The presence of recombinant Cu/Zn-SOD with a relative molecular weight of 20.8 kDa was demonstrated in the lysates of the induced in SDS-PAGE. The recombinant Cu/Zn-SOD reacted with mouse and bovine antiserum samples in Western blot. The preservation of its immunoreactivity indicates that the recombinant Cu/Zn-SOD might be a good antigen for development of vaccine and diagnostic reagents.
Pages: 46-51
Year: 2014 Issue: 2
Journal: Chinese Journal of Animal Infectious Diseases
Keyword: 超氧化物歧化酶; 布鲁氏菌; 体外表达; 特性分析; 流产; 表达产物; 重组; 免疫原性; 疫苗开发; 分子量;
Abstract: Immunoreactive cytosolic Cu/Zn superoxide dismutase(Cu/Zn-SOD) of Brucella abortus was expressed and characterized in the present study. The gene encoding Cu/Zn-SOD was synthesized using two-round of PCR procedure. The Cu/Zn-SOD gene was cloned into pET-22b(+) vector for expression. The presence of recombinant Cu/Zn-SOD with a relative molecular weight of 20.8 kDa was demonstrated in the lysates of the induced in SDS-PAGE. The recombinant Cu/Zn-SOD reacted with mouse and bovine antiserum samples in Western blot. The preservation of its immunoreactivity indicates that the recombinant Cu/Zn-SOD might be a good antigen for development of vaccine and diagnostic reagents.
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