Identification of a Herbicide Safener AD-67 Inducible cDNA in Rice
Author(s): YIN De-suo, SUN Xiao-qiong, LI Ke, WANG Shi-quan, DENG Qi-ming, LI Ping
Pages: 235-238
Year: 2007 Issue: 3
Journal: RICE SCIENCE
Keyword: rice; chemical induction; cDNA; mRNA differential display; herbicide safener; inducible gene;
Abstract: A herbicide safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The transcript was increased 6 h after sprayed with the safener solution, and 4 days later, the expression still could be detected. The fragment was recycled from the poly-gel and sequenced, and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice database, and the amino acid sequence was 60-84% homologous to those of the Yippee genes in several eukaryotes. The fragment was extended to the whole long cDNA, and thus a primer pair was designed. RT-PCR analysis for the designed primer supported the induction result.
Pages: 235-238
Year: 2007 Issue: 3
Journal: RICE SCIENCE
Keyword: rice; chemical induction; cDNA; mRNA differential display; herbicide safener; inducible gene;
Abstract: A herbicide safener AD-67 inducible cDNA was identified in an indica rice variety 9311 by mRNA differential display. The transcript was increased 6 h after sprayed with the safener solution, and 4 days later, the expression still could be detected. The fragment was recycled from the poly-gel and sequenced, and homologous analysis revealed the cDNA was 100% identical to some ESTs and cDNAs in rice database, and the amino acid sequence was 60-84% homologous to those of the Yippee genes in several eukaryotes. The fragment was extended to the whole long cDNA, and thus a primer pair was designed. RT-PCR analysis for the designed primer supported the induction result.
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