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Effect of carbon disulfide on mitochondrial apoptosis-related proteins in cytoplasm of testicular tissue among male rats
Author(s): 
Pages: 730-733
Year: Issue:  10
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  Carbon disulfideSpermatogenic cellMitochondrionApoptosis;
Abstract: Objective To investigate the role of mitochondrial pathway in the apoptosis of spermatogenic cells induced by inhalation of carbon disulfide in male rats.Methods Twenty-four male Sprague-Dawley rats (clean grade) were divided into four groups according to their body weights:three CS2 exposure groups (CS2 concentrations:50,250,and 1250 mg/m3) and a control group.The rats in CS2 exposure groups were exposed to CS2 by static inhalation for 10 weeks (2 h/d,5 d/w),while the rats in control group were exposed to air.Then,all rats were sacrificed by decapitation; testicular tissues were collected,and cytoplasmic proteins were extracted;the levels of apoptosis-inducing factor (AIF),cytochrome c (cyto c),Bcl-2,Bax,procaspase-9,and procaspase3 were measured by Western blot,and the activities of caspase-9 and caspase-3 were measured using a test kit.Results Compared with the control group,all CS2 exposure groups had significantly increased levels of cyto c in the cytoplasm of testicular tissue (P<0.05); in the 250 mg/m3 CS2 exposure group,the Bax/Bcl-2 ratio and activities of caspase-9 and caspase-3 increased significantly (P<0.05),and the content of procaspase-9 and procaspase-3 decreased significantly (P<0.05); in the 1250 mg/m3 CS2 exposure group,the relative expression levels of Bax and AIF in cytoplasm increased significantly (P<0.05),and the expression level of Bcl-2 decreased significantly (P<0.05).Conclusion Mitochondrial pathway plays an important role in the CS2-induced apoptosis of spermatogenic cells in testicular tissue among male rats.
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