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Effect of carb on disulfide on mitochondrial respiratory chain in spermatogenic cells in male rats
Author(s): 
Pages: 726-729
Year: Issue:  10
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  Carbon disulfideSpermatogenic cellMitochondrionRespiratory chain enzyme complex;
Abstract: Objective To investigate the effect of carbon disulfide (CS2) on the mitochondrial respiratory chain in testicular spermatogenic cells in male rats and to explore the possible mechanism of reproductive system damage caused by CS2 in male rats.Methods Twenty-four male Sprague-Dawley rats (clean grade)were randomly divided into four groups:three CS2 exposure groups (CS2 concentrations:50,250,and 1250 mg/m3)and a control group.The rats in CS2 exposure groups were exposed to CS2 by static inhalation for 10 weeks (2 h/d,5 d/w),while the rats in control group were exposed to air.Then,all rats were sacrificed by decapitation;testicular tissues were collected,and mitochondrial protein in spermatogenic cells were extracted; the levels of mitochondrial respiratory chain enzyme complex Ⅰ~Ⅴ were measured by enzyme-linked immunosorbent assay.Results Compared with the control group,all CS2 exposure groups had significantly increased levels of mitochondrial respiratory chain enzyme complex Ⅰ~Ⅴ in spermatogenic cells (P<0.05).There were no significant differences in the levels of respiratory chain enzyme complex Ⅰ~Ⅳ between the CS2 exposure groups (P<0.05),but the level of respiratory chain enzyme complex Ⅴ rose significantly as the concentration of CS2 increased (P<0.05).Conclusion Various levels of CS2 exposure may increase the levels of mitochondrial respiratory chain enzyme complex in testicular spermatogenic cells among male rats,thus affecting the normal oxidative phosphorylation in mitochondria.
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