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Investigation on biological characteristics of CD133 + cancer stem cells in human laryngeal carcinoma Hep-2 cell line
Pages: 578-583
Year: Issue:  7
Journal: Chinese Journal of Otorhinolaryngology Head and Neck Surgery

Keyword:  Laryngeal neoplasmsCell linetumorNeoplastic stem cellsNeoplasm invasivenessClone cellsRadiation toleranceDrug resistanceneoplasm;
Abstract: Objective To investigate the biology characters of CD133 + cancer stem cells from Hep-2 cell line.Methods The Flow cytometry was applied to purify CD133 + cells from Hep-2 cell line.The sorted CD133 + cells were cultured in RPMII640.The ability of migration,invasion and clonality of CD133 + cells were performed to characterize the properties of the cells.CD133 + cells and CD133-control cells were treated with paclitaxel and exposed respectively to X-rays emitted by linear accelerator with a dose of 10 Gy.The surviving rates and growth inhibition ratio of cells in two groups were detected with MTT assay to observe the resistance to irradiation and chemotherapy in CD133 + cells.Results The percentage of CD133+ cells in the unsorted and the sorted cells were 3.1% ±0.2% and 90.2% ±5.5%,respectively.CD133+ cells showed the higher proliferation and colony ability than CD133-cells.The numbers of CD133 + and CD133-cells that passed the membrane of Transwell chamber were 526 ± 39 and 220 ± 20 respectively(t =22.08,P < 0.001).The colony forming units of three passages were 30.0% ± 4.7%,32.2% ±3.6%,32.7% ±3.4% in CD133+ cells and 15.2% ±2.2%,12.0% ±2.5%,13.8% ±3.3%in CD133-cells.There were statistic difference between two groups(t =8.99,t =14.66,t =12.69,P <0.01).At 24,48,and 72 hours of treatment with paclitaxel,the cell surviving rates of CD133 + cells were 90.1% ±5.9%,85.1% ±7.1% and 70.3% ±6.4% and lower than those of CD133-cells,respectively (t =5.24,t =8.18,t =8.14,P < 0.01).After radiotherapy,growth inhibition ratio of CD133 + and CD133-cells were 30.0% ±7.1% and 55.0% ±6.3%(t =8.30,P<0.01).Conclusions CD133+ cells exist in a small proportion in Hep-2 cell line and they show the properties of cancer stem cells,with the resistance to irradiation and chemotherapy.
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