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zhu hou yan sheng ying guang jian ce gao xiao ye xiang se pu fa ce ding ban li zhong de an ji suan
Author(s): Wang Weidong
Pages: 23-
29
Year: 1991
Issue:
2
Journal: Nonwood Forest Research
Keyword: 高效液相色谱; 氨基酸; 柱后衍生; 荧光检测; 板栗;
Abstract: 本文介绍了测定板栗中氨基酸的高效液相色谱法。样品水解产物中的氨基酸采用离子交换法分离、梯度洗脱、OPA(邻苯二甲醛)柱后衍生,然后再用荧光测法检测(Ex348nm,Em450nm)。17种氨基酸在50分钟内得到完全的分离。方法的标准偏差在0.16%以内,变异系数为2.8—14%(n=4)。
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