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Detection of Infectious Bronchitis Virus and Infectious Laryngotracheitis Virus by Two-temperature PCR
Author(s): XIE Zhiqin, XIE Zhixun, PANG Yaoshan, LIU Jiabo, DENG Xianwen, LIAO Min
Pages: 152-
155
Year: 2001
Issue:
2
Journal: GUANGXI SCIENCES
Keyword: 鸡传染性支气管炎病毒; 鸡传染性喉气管炎病毒; 二温式多重聚合酶链式反应; 检测;
Abstract: 为了简化聚合酶链反应(PCR)程序和加快检测速度,将二温式PCR与多重PCR结合起来,建立一种同时检测鸡传染性支气管炎病毒(IBV)和鸡传染性喉气管炎病毒(ILTV)的二温式多重PCR技术.试验根据IBV和ILTV的基因文库,设计2对分别与IBV和ILTV某段基因序列互补的引物,用这2对引物对同一样品中的IBV、ILTV核酸模板进行二温式多重PCR扩增,结果均同时得到2条特异性大小与实验设计相符的1720bp(IBV)和647bp(ILTV)的扩增带,而对其他6种禽病病原的扩增结果均为阴性;敏感性测定结果表明,该二温式多重PCR技术能检出10pg的IBV RNA模板和10pg的ILTV DNA模板.
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