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The Evaluation and Optimization of the Early Embryos' Development of Mouse
Author(s): 
Pages: 147-152
Year: Issue:  2
Journal: ACTA LASER BIOLOGY SINICA

Keyword:  类胰岛素生长因子(IGF)上皮生长因子(EGF)血小板激活因子(PAF);
Abstract: Embryos were collected at the zygote or 2-cell.zygote s were produced by fertilization in situ(ISV)or in vitro(IVF).Two-cell-stage e mbryos had a high rate of development to the blastocyst stage across the embryo concentration range of 1/μl~1/1000?μl.By contrast,zygotes produced by either I SF or IVF were adversely affected by reduced the embryo concentration over this range(p<0.01),with approximate 82.5% of ISF zygotes developing to blastocysts at highest concentration but only 22.3% at the lowest.For IVF zygotes the corre sponding results were 46.3% and 5.2%.Compared 2-cell embryos with ISF and IVF g roups,the number of cell in each blastocyst was significantly higher.Platelet-a ctivating factor(PAF) supplymentation of media caused a significant increase in the rate of blastocyst development of IVF zygotes at embryo concentration of 1/1 0?μl(10ng/ml) and 1/100μl(100ng/ml).Insulin-like growth factor(IGF)(1 0? ng/ml)also stimulated development of IVF zyogtes when cultured at an embryo conc entration of 1/10μl.Eodermal growth factor was without effect over range of 1 ~1000ng/ml.Supplymentation of media with both PAF and IGF-Ⅰ gave no additio na l benefit over that caused by IGF-Ⅰ along,but this treatment was marginally be tter(p<0.05) than PAF treatment along.The results show that factors necessary f or normal embryo development are diluted to suboptimal levels during culture at low embryo concentration.The ability of PAF,IGF-Ⅰ to partially compensate for the adverse effects of low embryo concentration during culture is consistent wit h having roles as autocrine embryotrophic factors.
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