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Experimental studies on the ABCG2 expression and chemoresistance of suspension sphere-forming PC-3 cells
Author(s): ZHANG Bo, FAN Xin-lan, LIN Tian-xin, XU Ke-wei, HUANG Hai, HUANG Jian
Pages: 787-
791
Year: 2012
Issue:
11
Journal: New Medicine
Keyword: 前列腺癌; 悬浮成球细胞; ATP结合盒膜转运蛋白2; 化学治疗耐药性;
Abstract: 目的:探讨人雄激素非依赖性前列腺癌PC-3悬浮成球细胞中ATP结合盒膜转运蛋白G超家族成员2 (ABCG2)的表达及其对化学治疗的耐药性.方法:体外无血清悬浮培养PC-3细胞,逆转录定量-PCR检测PC-3悬浮成球细胞中ABCG2 mRNA的表达,采用MTT法检测PC-3悬浮成球细胞对顺铂的耐药性,计算半数抑制浓度(IC50),并与PC-3贴壁细胞作对比.结果:PC-3悬浮成球细胞可以在无血清培养条件下生存并形成悬浮细胞球,PC-3悬浮成球细胞中ABCG2 mRNA的相对表达水平是PC-3贴壁细胞的33.98倍(P<0.05),其IC50是PC-3贴壁细胞的4.26倍(P<0.05).结论:人PC-3悬浮成球细胞中ABCG2表达水平较高,该类细胞对化学治疗具有较强耐药性.
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