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Application of qPCR-HRM curve analysis and common-PCR with DNA sequencing in detection of EGFR mutations in glioma tissues
Author(s): XIONG Liang, LIANG Chao-feng, CHEN Chuan, LUO Lun, LING Cong, QUAN Feng, CAI Mei-qin, SHI Zhi-dong
Pages: 327-
330
Year: 2012
Issue:
5
Journal: New Chinese Medicine
Abstract: 目的:比较实时聚合酶链反应-高分辨率融解(qPCR-HRM)曲线分析技术和普通PCR法加直接测序法检测胶质瘤患者EGFR基因突变类型,探讨适用于临床的EGFR基因突变检测方法,为胶质瘤患者术后放射、化学治疗及预后判断研究提供可靠的依据.方法:用普通PCR加测序法与qPCR-HRM曲线分析技术检测胶质瘤患者EGFR基因突变类型,检测结果比较采用卡方检验.结果:两种方法检测EGFR外显子19基因突变结...
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