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The investigation of DNA double-strand breaks of lymphocytes in workers exposed to lead and DNA double-strand breaks of human lymphocytes induced by lead in vitro
Author(s): LUO Ming-jin, HU Xiao-jun, LI Chen, YANG Jun, WANG Jing
Pages: 216-
219
Year: 2012
Issue:
3
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword: Lead; Lymphocytes; DNA damage;
Abstract: 目的 用γH2AX识别抗体流式细胞术研究铅暴露致人体外周血淋巴细胞DNA双链断裂(DSBs)作用.方法 选取某蓄电池厂工人36人为铅接触组,其中高浓度组15人,低浓度组21人;同时选择厂外无职业性铅接触70人为对照组,取外周静脉血提取淋巴细胞,利用流式细胞术检测γH2AX,分析淋巴细胞中DNA DSBs水平;不同剂量、时间下醋酸铅染毒健康人外周血淋巴细胞,利用流式细胞术检测γH2AX,分析淋巴细胞...
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