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The effects of oxidative damage induced by organic oxidant t-BHP on cochlear hair cells
Author(s): 
Pages: 186-189
Year: Issue:  3
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  Tert-butylhydroperoxideNoiseHair cellsOxidative stressReactive oxygen species;
Abstract: 目的 研究有机氧化剂叔丁基过氧化氢(t-BHP)体外模拟噪声对耳蜗毛细胞的氧化损伤.方法 用t-BHP染毒,设置从30~4000 μmol/L8个染毒浓度对耳蜗毛细胞(HEI-OC1)细胞染毒12h,绘制100 μmol/L浓度组染毒时间(1~96 h)与耳蜗毛细胞存活率曲线;采用台盼蓝染色法检测细胞存活率,噻唑蓝(MTT)试验检测细胞增殖能力的改变,2’-7’-二氯荧光黄双乙酯(DCFH-DA)...
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