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The pathological changes and apoptosis of spleen lymphocytes in mice induced by electromagnetic pulses
Author(s): ZHANG Hua-wei, ZHOU Yong-chun, GUO Juan, ZHANG Yan-jun, LIU Jun-ye, GUO Guo-zhen
Pages: 168-
171
Year: 2012
Issue:
3
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases
Keyword: Electromagnetic fields; Spleen; Lymphocytes; Apoptosis;
Abstract: 目的 研究电磁脉冲(electromagnetic pulse,EMP)对小鼠脾脏淋巴细胞病理变化和凋亡的影响.方法 用场强为200 kV/m的EMP,辐照雄性BALB/c小鼠,每天照射400次,连续照射7d,照后1、3、7、14、28d共5个时间点处死小鼠取脾脏,观察其病理改变;处死后称取小鼠的体重和脾脏的重量,计算脾脏指数;然后提取淋巴细胞进行计数;通过流式细胞仪检测淋巴细胞的凋亡和周期;通过...
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