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The effects of complement C3f segment on expression and secretion of collagen Ⅰ, Ⅲ and transforming growth factor-β1 in human embryonic lung fibroblast
Author(s): 
Pages: 61-63
Year: Issue:  1
Journal: Chinese Journal of Industrial Hygiene and Occupational Diseases

Keyword:  FibrobastsComplementCollagenTransforming growth factor beta;
Abstract: 目的 观察补体片段C3f对人胚肺成纤维细胞(MRC-5)表达和分泌Ⅰ、Ⅲ型胶原及转化生长因子(TGF)-β1的影响.方法 将人工合成的17肽片断C3f加入培养基中刺激MRC-5细胞,通过细胞免疫组化和ELISA法检测Ⅰ、Ⅲ型胶原及TGF-β1细胞内、外表达情况.结果 随着C3f浓度的增加,MRC-5细胞上清液中Ⅰ、Ⅲ型胶原和TGF-β1的表达量逐渐降低,与对照组相比,差异有统计学意义(P<0.05...
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