Author(s): REN Zuoran, ZHANG Yuguang, JIANG Zuo, JIN Junhua, REN Fazheng
Pages: 55-58
Year: 2011 Issue:  22
Journal: China Dairy Cattle
Keyword:  Bacillus Brevis; Tyrothricin; Dissolved oxygen; Optimization; Two stages with different oxygen supplement;
Abstract: 本研究通过优化溶氧条件，对短芽孢杆菌（BacillusBrevis）（ATCC8185）进行分段培养，提高发酵液中短杆菌素产量。以析因试验确定菌体生成阶段培养条件为装液量35mL／100mL，摇床转速120r／min；以二次饱和D-最优设计试验确定短杆菌素合成阶段培养条件为：培养36h后静置培养。通过对溶氧条件的优化，发酵液中短杆菌素的产量达到616．93＋9．72μg／mL，比优化前提高了89％...